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GBP1 Polyclonal antibody

GBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15303-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

Guanylate-binding protein 1, Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman placenta tissue, human brain tissue, HUVEC cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GBP1 Ready-To-Use IHC Kit. GBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15303-1-AP targets GBP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, RIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 GBP1 fusion protein Ag7562 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTP binding protein 1
别名 Guanylate-binding protein 1, Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1
计算分子量 68 kDa
观测分子量 67 kDa
GenBank蛋白编号BC002666
基因名称 GBP1
Gene ID (NCBI) 2633
RRIDAB_2247448
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32455
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP.

 What is the molecular weight of GBP1?

The molecular weight of GBP1 is 68kDa.

 What is the cellular localization of GBP1?

GBP1 is predominantly cytosolic, but under certain conditions is farnesylated and associated with intracellular membranes such as the Golgi (PMID: 15937107).

 What is the role of GBP1 in immunity?

GBP1 is induced in response to type I and type II IFN and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica (PMID: 21142871). These anti-pathogenic functions involve the recruitment to vacuolar compartments for the destruction of the pathogen, the assembly of innate immune complexes such as the inflammasome, or the initiation of autophagy.

 What is the role of GBP-1 in colorectal carcinoma?

In colorectal carcinoma (CRC), the presence of tumor-infiltrating T helper type 1 (Th1) cells participates in anti-tumoral responses and an improved clinical outcome (PMID: 17008531, 20927778). IFN promote the Th1 immune response, as well as GBP-1 expression. In CRC, GBP-1 expression acts as a tumor suppressor, where inhibition of tumor growth by GBP-1 is the result of its strong antiangiogenic activity as well as its direct anti-tumorigenic effect on tumor cells and is correlated with a better outcome (PMID: 23042300).


实验方案

Product Specific Protocols
WB protocol for GBP1 antibody 15303-1-APDownload protocol
IHC protocol for GBP1 antibody 15303-1-APDownload protocol
IF protocol for GBP1 antibody 15303-1-APDownload protocol
IP protocol for GBP1 antibody 15303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Immunol

Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.

Authors - Michal P Wandel
  • KD Validated
humanWB

Nat Cell Biol

LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.

Authors - Gaopeng Li
humanWB

Cell Host Microbe

GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8.

Authors - Michal P Wandel
humanWB,IP

J Med Virol

Guanylate-binding protein 1 inhibits Hantaan virus infection by restricting virus entry

Authors - Tianle Gu
humanWB

Nat Commun

Mechanistic insight into bacterial entrapment by septin cage reconstitution.

Authors - Damián Lobato-Márquez
humanWB,IHC,IF

J Transl Med

Function and mechanism of GBP1 in the development and progression of cervical cancer

Authors - Senyu Wang
  • KD Validated
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