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GLRA2 Polyclonal antibody

GLRA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13831-1-AP

Print datasheet

Synonyms

GLR, GLRA2, glycine receptor, alpha 2

验证数据展示

  • WB analysis of PBMC cells using 13831-1-AP

    WB analysis of PBMC cells using 13831-1-AP

    WB result of GLRA2 antibody (13831-1-AP, 1:600) with PBMC cells. Proteintech αGLRA2 13831-1-AP in 10% BSA / TBS 0.05% Tween-20 O/N @ 4°C Goat anti-rabbit HRP 1:10000 in 5% milk / TBS 0.05% Tween-20 1h @ RT.

  • IHC staining of mouse brain using 13831-1-AP

    IHC staining of mouse brain using 13831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 13831-1-AP

    IHC staining of mouse brain using 13831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 13831-1-AP

    WB analysis using 13831-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13831-1-AP (GLRA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPBMC cells, K-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13831-1-AP targets GLRA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GLRA2 fusion protein Ag4743 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycine receptor, alpha 2
Synonyms GLR, GLRA2, glycine receptor, alpha 2
Calculated Molecular Weight 452 aa, 52 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC032864
Gene Symbol GLRA2
Gene ID (NCBI) 2742
RRIDAB_2877980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23416
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

实验方案

Product Specific Protocols
WB protocol for GLRA2 antibody 13831-1-APDownload protocol
IHC protocol for GLRA2 antibody 13831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols