验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GOLM1 Ready-To-Use IHC Kit. GOLM1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15126-1-AP targets GP73/GOLPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | GP73/GOLPH2 fusion protein Ag7207 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | golgi membrane protein 1 |
| 别名 | GOLM1, GOLPH2, GP73, GOLM 1, golgi protein 73kD |
| 计算分子量 | 45 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC001740 |
| 基因名称 | GP73 |
| Gene ID (NCBI) | 51280 |
| RRID | AB_2113330 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NBJ4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IHC protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IF protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IP protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Pathog GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.
| ||
J Cell Sci Rapid affinity purification of intracellular organelles using a twin strep tag. | ||
Chemistry Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction | ||
Int J Med Sci Causal Association of Golgi Protein 73 With Coronary Artery Disease: Evidence from Proteomics and Mendelian Randomization | ||
J Cancer GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.
| ||
Curr Alzheimer Res RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase. |
