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验证数据展示

  • WB analysis using 15126-1-AP

    WB analysis using 15126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton and nuclei were stained with CL594-phalloidin and DAPI, respectively.

  • WB analysis using 15126-1-AP

    WB analysis using 15126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15126-1-AP

    IP experiment of HeLa using 15126-1-AP

    IP result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • IHC staining of human stomach cancer using 15126-1-AP

    IHC staining of human stomach cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human liver cancer using 15126-1-AP

    IHC staining of human liver cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human liver cancer using 15126-1-AP

    IHC staining of human liver cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

GP73/GOLPH2 Polyclonal antibody

GP73/GOLPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15126-1-AP

产品说明书

引用文献(17)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

GOLM1, GOLPH2, GP73, GOLM 1, golgi protein 73kD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLM1 Ready-To-Use IHC Kit. GOLM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15126-1-AP targets GP73/GOLPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 GP73/GOLPH2 fusion protein Ag7207 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi membrane protein 1
别名 GOLM1, GOLPH2, GP73, GOLM 1, golgi protein 73kD
计算分子量 45 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC001740
基因名称 GP73
Gene ID (NCBI) 51280
RRIDAB_2113330
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBJ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IHC protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IF protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IP protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation

Authors - Wanxin Guo
View Article
humanWB

PLoS Pathog

GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.

Authors - Xuewu Zhang
  • KD Validated
View Article
humanWB

J Cell Sci

Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors - Jian Xiong
View Article
humanIF

Chemistry

Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction

Authors - Sayan Kundu
View Article
mouseWB,IF

Int J Med Sci

Causal Association of Golgi Protein 73 With Coronary Artery Disease: Evidence from Proteomics and Mendelian Randomization

Authors - Yi-Fen Lin
View Article
humanWB,IHC,IF

J Cancer

GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.

Authors - Jia-Zhou Ye
  • KD Validated
View Article