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验证数据展示

  • WB analysis using 15126-1-AP

    WB analysis using 15126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton and nuclei were stained with CL594-phalloidin and DAPI, respectively.

  • WB analysis using 15126-1-AP

    WB analysis using 15126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15126-1-AP

    IP experiment of HeLa using 15126-1-AP

    IP result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • WB analysis of HeLa using 15126-1-AP

    WB analysis of HeLa using 15126-1-AP

    WB result of GP73/GOLPH2 antibody (15126-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GP73/GOLPH2 transfected HeLa cells.

  • IHC staining of human stomach cancer using 15126-1-AP

    IHC staining of human stomach cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human liver cancer using 15126-1-AP

    IHC staining of human liver cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human liver cancer using 15126-1-AP

    IHC staining of human liver cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

GP73/GOLPH2 Polyclonal antibody

GP73/GOLPH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15126-1-AP

产品说明书

引用文献(23)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

GOLM1, GOLPH2, GP73, C9orf155, Golgi membrane protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLM1 Ready-To-Use IHC Kit. GOLM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15126-1-AP targets GP73/GOLPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag7207

Product name: Recombinant human GOLM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-400 aa of BC001740

Sequence: SRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi membrane protein 1
别名 GOLM1, GOLPH2, GP73, C9orf155, Golgi membrane protein 1
计算分子量 45 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC001740
基因名称 GP73
Gene ID (NCBI) 51280
RRIDAB_2113330
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBJ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

实验方案

Product Specific Protocols
IF protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IHC protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IP protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
WB protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Signal Transduct Target Ther

GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis.

Authors - Yang Pu
View Article
mouse,humanWB,IF

Mol Cancer

GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis

Authors - Linfei Huang
View Article
mouse,humanWB,IHC,IF

Clin Mol Hepatol

GOLM1 promotes cholesterol gallstone formation via ABCG5-mediated cholesterol efflux in MASH livers

Authors - Yi-Tong Li
  • KD Validated
View Article
humanWB

J Extracell Vesicles

A Unique Intercellular Feedforward Loop From HK1 to TGF-β1 Promotes the Progression of Hepatocellular Carcinoma.

Authors - Qi-tao Chen
View Article
mouse,humanIF

J Adv Res

The GOLM1-ACLY pathway regulates macrophage-secreted EFEMP1 via H3K27ac modifications to drive tumor progression

Authors - Lan Zhen
View Article

Mol Cell

LZTFL1 rewires NADPH-glutathione metabolism to amplify ferroptosis.

Authors - Xiangfei Xue
View Article
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