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Syntabulin Polyclonal antibody

Syntabulin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16972-1-AP

Print datasheet

Synonyms

Syntaxin-1-binding protein, SYBU, KIAA1472, GOLSYN, Golgi-localized syntaphilin-related protein

验证数据展示

  • WB analysis of HeLa using 16972-1-AP

    WB analysis of HeLa using 16972-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16972-1-AP (Syntabulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of rat brain using 16972-1-AP

    IF Staining of rat brain using 16972-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 16972-1-AP (Syntabulin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 16972-1-AP

    IF Staining of HeLa using 16972-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16972-1-AP (Syntabulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16972-1-AP targets Syntabulin in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Syntabulin fusion protein Ag9344 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Golgi-localized protein
Synonyms Syntaxin-1-binding protein, SYBU, KIAA1472, GOLSYN, Golgi-localized syntaphilin-related protein
Calculated Molecular Weight 662 aa, 72 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC012082
Gene Symbol GOLSYN
Gene ID (NCBI) 55638
RRIDAB_1939519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Syntabulin antibody 16972-1-APDownload protocol
IF protocol for Syntabulin antibody 16972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Hum Mol Genet

Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.

Authors - Saravanan Gunaseelan
View Article
mouse,humanWB,IF

Cell Death Discov

Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B

Authors - Pingyang Ke
View Article
mouseWB

Neurobiol Dis

Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.

Authors - Qi Wang
View Article