- Featured Product
- KD/KO Validated
GPR161 Polyclonal antibody
GPR161 Polyclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, Canine and More (3)
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IF detected in | MDCK cells, ARPE-19 cells, C2C12 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13398-1-AP targets GPR161 in WB, IF, ELISA applications and shows reactivity with human, mouse, Canine samples.
| Tested Applications | IF/ICC, WB, ELISA |
| Cited Applications | IF, WB |
| Tested Reactivity | human, mouse, Canine |
| Cited Reactivity | human, mouse, chicken, zebrafish, pig |
| Immunogen | GPR161 fusion protein Ag4221 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | G protein-coupled receptor 161 |
| Synonyms | G protein coupled receptor 161, G protein coupled receptor RE2, GPR161, RE2 |
| Calculated Molecular Weight | 529 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC028163 |
| Gene Symbol | GPR161 |
| Gene ID (NCBI) | 23432 |
| RRID | AB_2113965 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N6U8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band probably represents a modified version of GPR161 (PMID: 18250320).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR161 antibody 13398-1-AP | Download protocol |
| IF protocol for GPR161 antibody 13398-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Primary cilium remodeling mediates a cell signaling switch in differentiating neurons. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Nat Commun Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling. | ||
Dev Cell Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling. | ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. |
