验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IF/ICC detected in | MDCK cells, ARPE-19 cells, C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13398-1-AP targets GPR161 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, canine |
| 文献引用反应性 | human, mouse, pig, chicken, zebrafish, bovine |
| 免疫原 | GPR161 fusion protein Ag4221 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 161 |
| 别名 | G-protein coupled receptor RE2, G-protein coupled receptor 161 |
| 计算分子量 | 529 aa, 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC028163 |
| 基因名称 | GPR161 |
| Gene ID (NCBI) | 23432 |
| RRID | AB_2113965 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N6U8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band probably represents a modified version of GPR161 (PMID: 18250320).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR161 antibody 13398-1-AP | Download protocol |
| IF protocol for GPR161 antibody 13398-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Primary cilium remodeling mediates a cell signaling switch in differentiating neurons. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Nat Commun Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling. | ||
Dev Cell Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling. | ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. |
