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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    WB analysis using 67763-1-Ig (same clone as 67763-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67763-1-Ig (GPX4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    IHC staining of human liver cancer using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67763-1-Ig (GPX4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67763-1-Ig (same clone as 67763-1-PBS)

    IF Staining of HeLa using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67763-1-Ig (same clone as 67763-1-PBS)

    WB analysis of HEK-293 using 67763-1-Ig (same clone as 67763-1-PBS)

    WB result of GPX4 antibody (67763-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 transfected HEK-293 cells. This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    IF Staining of mouse testis using 67763-1-Ig (same clone as 67763-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GPX4 antibody (67763-1-Ig, Clone: 3F5G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67763-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GPX4 Monoclonal antibody, PBS Only

GPX4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67763-1-PBS

产品说明书

CloneNo. 3F5G5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

3F5G5, EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67763-1-PBS targets GPX4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken, zebrafish, hamster, dog
免疫原 GPX4 fusion protein Ag30650 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glutathione peroxidase 4 (phospholipid hydroperoxidase)
别名 3F5G5, EC:1.11.1.12, EC:1.11.1.9, GPx 4, GPx-4
观测分子量 20-23 kDa
GenBank蛋白编号BC021567
基因名称 GPX4
Gene ID (NCBI) 2879
RRIDAB_2909469
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36969
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.