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GR repeat Polyclonal antibody

GR repeat Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB,ELISA and More (3)

Conjugate

Unconjugated

Cat No : 23978-1-AP

Print datasheet

Synonyms

C9RAN, GGGCC, GR repeat, GRGRGRGRG, GRGRGRGRGR, Poly-GR



经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23978-1-AP targets GR repeat in WB, IHC, IF, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Applications WB,ELISA
Cited Applications Dot Blot, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish, Drosophila
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GR repeat
Synonyms C9RAN, GGGCC, GR repeat, GRGRGRGRG, GRGRGRGRGR, Poly-GR
Gene Symbol
Gene ID (NCBI)
RRIDAB_2879387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GR repeated sequence. The recombinant proteins GST-SNRPD1 and 6*His tag- SNRPD1 which contains the sequence "GRGRGRGRGRGRGRGRGR" were used to detect this antibody. Both proteins can be recognized by the antibody 23978-1-AP.

实验方案

Product Specific Protocols
WB protocol for GR repeat antibody 23978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

The C9orf72 repeat expansion disrupts nucleocytoplasmic transport.

Authors - Ke Zhang
DrosophilaWB

Science

C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins.

Authors - Sarah Mizielinska
humanIF

Cell

Stress Granule Assembly Disrupts Nucleocytoplasmic Transport.

Authors - Ke Zhang
  • KD Validated
humanIHC

Nat Neurosci

RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats.

Authors - Shizuka B Yamada
zebrafishDot Blot

Acta Neuropathol

A zebrafish model for C9orf72 ALS reveals RNA toxicity as a pathogenic mechanism.

Authors - Bart Swinnen
humanIHC

Acta Neuropathol

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Authors - Yuichi Riku