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GRB2 Polyclonal antibody

GRB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10254-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Growth factor receptor-bound protein 2, Grb33, EGFRBP GRB2, ASH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, rat brain tissue, rat spleen tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GRB2 Ready-To-Use IHC Kit. GRB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10254-2-AP targets GRB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 GRB2 fusion protein Ag0395 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 2
别名 Growth factor receptor-bound protein 2, Grb33, EGFRBP GRB2, ASH
计算分子量 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC000631
基因名称 GRB2
Gene ID (NCBI) 2885
RRIDAB_2263577
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62993
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

实验方案

Product Specific Protocols
WB protocol for GRB2 antibody 10254-2-APDownload protocol
IHC protocol for GRB2 antibody 10254-2-APDownload protocol
IF protocol for GRB2 antibody 10254-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Mol Ther Nucleic Acids

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Authors - Lingjiao Meng
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
humanIHC

Cancer Lett

Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2.

Authors - Jie Lv
humanWB,IHC

Oncotarget

mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer.

Authors - Xinjing Wang
humanWB

Food Funct

Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel

Authors - Mengqi Zhang

Cancer Lett

Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma.

Authors - Xinjing Wang
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