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验证数据展示

  • WB analysis using 28482-1-AP

    WB analysis using 28482-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28482-1-AP (GRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28482-1-AP

    IHC staining of human lung cancer using 28482-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 28482-1-AP

    IHC staining of human lung cancer using 28482-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28482-1-AP (GRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRP Polyclonal antibody

GRP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28482-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Synonyms

BN, gastrin releasing peptide, GRP, GRP 10, preproGRP, proGRP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

28482-1-AP targets GRP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen GRP fusion protein Ag29357 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gastrin-releasing peptide
Synonyms BN, gastrin releasing peptide, GRP, GRP 10, preproGRP, proGRP
Calculated Molecular Weight 16 kDa
Observed Molecular Weight16-18 kDa
GenBank Accession NumberBC004488
Gene Symbol GRP
Gene ID (NCBI) 2922
RRIDAB_2881152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07492
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRP antibody 28482-1-APDownload protocol
IHC protocol for GRP antibody 28482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Ann N Y Acad Sci

The von Economo neurons in the frontoinsular and anterior cingulate cortex.

Authors - John M Allman
View Article
humanIHC

Front Immunol

A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma

Authors - Muhammad Khan
View Article