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- KD/KO Validated
GRP78/BIP Polyclonal antibody
GRP78/BIP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
625
验证数据展示
经过测试的应用
Positive WB detected in | BxPC-3 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, HT-29 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue, mouse kidney tissue, rat brain tissue, mouse heart tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11587-1-AP targets GRP78/BIP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, monkey, chicken, fish, carp, medaka embryos, deer |
Immunogen | GRP78/BIP fusion protein Ag2188 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
Synonyms | BIP, GRP78, HSPA5, GRP 78, Endoplasmic reticulum chaperone BiP |
Calculated Molecular Weight | 78 kDa |
Observed Molecular Weight | 70-78 kDa |
GenBank Accession Number | BC020235 |
Gene Symbol | GRP78/BIP |
Gene ID (NCBI) | 3309 |
RRID | AB_2119855 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P11021 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
IHC protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
IF protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
IP protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
FC protocol for GRP78/BIP antibody 11587-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science Translation from the 5' untranslated region shapes the integrated stress response. | ||
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. | ||
Acta Pharm Sin B SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation. | ||
J Pineal Res Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress |