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GRP78/BIP Monoclonal antibody, PBS Only

GRP78/BIP Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6F7

Cat No : 66574-1-PBS

Print datasheet

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

验证数据展示

  • WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

    IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

    IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP78/HSPA5 antibody included
  • IF Staining of HeLa using 66574-1-Ig (same clone as 66574-1-PBS)

    IF Staining of HeLa using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.

产品信息

66574-1-PBS targets GRP78/BIP in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IP, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen GRP78/BIP fusion protein Ag2188 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Synonyms BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC020235
Gene Symbol HSPA5
Gene ID (NCBI) 3309
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11021
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.