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验证数据展示

  • IF Staining of HepG2 using 60012-1-Ig (same clone as 60012-1-PBS)

    IF Staining of HepG2 using 60012-1-Ig (same clone as 60012-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 60012-1-Ig (same clone as 60012-1-PBS)

    WB analysis of human heart using 60012-1-Ig (same clone as 60012-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve ofMP50013-1, GRP94 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60012-2-PBS. Detection antibody: HRP-conjugated 60012-1-PBS. Standard: Ag1439. Range: 0.156-20 ng/mL..

GRP94 Monoclonal antibody, PBS Only (Detector)

GRP94 Monoclonal Antibody for WB, IF, IHC, Sandwich ELISA, Indirect ELISA
Cat No. 60012-1-PBS

产品说明书

CloneNo. 1H10B7

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat

应用

WB, IF, IHC, Sandwich ELISA, Indirect ELISA

别名

ECGP, Endoplasmin, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

产品信息

60012-1-PBS targets GRP94 as part of a matched antibody pair:

MP50013-1: 60012-2-PBS capture and 60012-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF, IHC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GRP94 fusion protein Ag1439 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock protein 90kDa beta (Grp94), member 1
别名 ECGP, Endoplasmin, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1
计算分子量 96 kDa
观测分子量 95 kDa
GenBank蛋白编号BC009195
基因名称 GRP94
Gene ID (NCBI) 7184
RRIDAB_2119056
偶联类型 Unconjugated
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP14625
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.