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GRP94 Monoclonal antibody, PBS Only (Detector)

GRP94 Monoclonal Antibody for WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H10B7

Cat no : 60012-1-PBS

Print datasheet

Synonyms

ECGP, Endoplasmin, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

验证数据展示

  • IF Staining of HepG2 using 60012-1-Ig (same clone as 60012-1-PBS)

    IF Staining of HepG2 using 60012-1-Ig (same clone as 60012-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60012-1-Ig (GRP94 antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 60012-1-Ig (same clone as 60012-1-PBS)

    WB analysis of human heart using 60012-1-Ig (same clone as 60012-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60012-1-Ig (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    IHC staining of human lung cancer using 60012-1-Ig (same clone as 60012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60012-1-Ig (GRP94 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve ofMP50013-1, GRP94 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60012-2-PBS. Detection antibody: HRP-conjugated 60012-1-PBS. Standard: Ag1439. Range: 0.156-20 ng/mL..

Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

产品信息

60012-1-PBS targets GRP94 as part of a matched antibody pair:

MP50013-1: 60012-2-PBS capture and 60012-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, IHC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GRP94 fusion protein Ag1439 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name heat shock protein 90kDa beta (Grp94), member 1
Synonyms ECGP, Endoplasmin, GP96, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC009195
Gene Symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP14625
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.