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GRP94 Monoclonal antibody, PBS Only (Capture)

GRP94 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G7C7

Cat no : 60012-2-PBS

Print datasheet

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

验证数据展示

  • WB analysis using 60012-2-Ig (same clone as 60012-2-PBS)

    WB analysis using 60012-2-Ig (same clone as 60012-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60012-2-Ig (GRP94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • FC experiment of HeLa using 60012-2-Ig (same clone as 60012-2-PBS)

    FC experiment of HeLa using 60012-2-Ig (same clone as 60012-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (60012-2-Ig, Clone:4G7C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve of MP50013-1, GRP94 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60012-2-PBS. Detection antibody: HRP-conjugated 60012-1-PBS. Standard: Ag1439. Range: 0.156-20 ng/mL..

  • IF Staining of HepG2 using 60012-2-Ig (same clone as 60012-2-PBS)

    IF Staining of HepG2 using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP94 antibody (60012-2-Ig, Clone: 4G7C7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

产品信息

60012-2-PBS targets GRP94 as part of a matched antibody pair:

MP50013-1: 60012-2-PBS capture and 60012-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GRP94 fusion protein Ag1439 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock protein 90kDa beta (Grp94), member 1
Synonyms HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC009195
Gene Symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14625
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).