验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, HEK-293 cells, HeLa cells, A549 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
产品信息
15113-1-AP targets GSK3B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GSK3B fusion protein Ag7101 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycogen synthase kinase 3 beta |
| 别名 | GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta |
| 计算分子量 | 433 aa, 48 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC000251 |
| 基因名称 | GSK3B |
| Gene ID (NCBI) | 2932 |
| RRID | AB_10640527 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49841 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSK3B antibody 15113-1-AP | Download protocol |
| IHC protocol for GSK3B antibody 15113-1-AP | Download protocol |
| IP protocol for GSK3B antibody 15113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway. | ||
J Ethnopharmacol Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway. | ||
Biochem Biophys Res Commun Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation. | ||
Biochem Biophys Res Commun NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer. | ||
Microvasc Res Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats. | ||
Cell Biol Int Identification and analysis of glycogen synthase kinase 3 beta1 interactome. |
