- Featured Product
- KD/KO Validated
GSK3B Polyclonal antibody
GSK3B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse ovary tissue, HEK-293 cells, HeLa cells, A549 cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 9 publications below |
产品信息
15113-1-AP targets GSK3B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | GSK3B fusion protein Ag7101 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | glycogen synthase kinase 3 beta |
Synonyms | GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta |
Calculated Molecular Weight | 433 aa, 48 kDa |
Observed Molecular Weight | 55-60 kDa |
GenBank Accession Number | BC000251 |
Gene Symbol | GSK3B |
Gene ID (NCBI) | 2932 |
RRID | AB_10640527 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P49841 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GSK3B antibody 15113-1-AP | Download protocol |
IHC protocol for GSK3B antibody 15113-1-AP | Download protocol |
IP protocol for GSK3B antibody 15113-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Oncol The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway. | ||
J Ethnopharmacol Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway. | ||
Biochem Biophys Res Commun Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation. | ||
Biochem Biophys Res Commun NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer. | ||
Microvasc Res Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats. | ||
Cell Biol Int Identification and analysis of glycogen synthase kinase 3 beta1 interactome. |