• Featured Product
  • KD/KO Validated

GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15113-1-AP

Print datasheet

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta



经过测试的应用

Positive WB detected inmouse ovary tissue, HEK-293 cells, HeLa cells, A549 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 9 publications below

产品信息

15113-1-AP targets GSK3B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen GSK3B fusion protein Ag7101 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycogen synthase kinase 3 beta
Synonyms GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta
Calculated Molecular Weight 433 aa, 48 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC000251
Gene Symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_10640527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49841
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.

实验方案

Product Specific Protocols
WB protocol for GSK3B antibody 15113-1-APDownload protocol
IHC protocol for GSK3B antibody 15113-1-APDownload protocol
IP protocol for GSK3B antibody 15113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway.

Authors - Chun Tang
humanWB

J Ethnopharmacol

Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway.

Authors - Tao Zheng
mouseWB

Biochem Biophys Res Commun

Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.

Authors - Jie Feng
humanWB

Biochem Biophys Res Commun

NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer.

Authors - Feikai Lin
humanWB

Microvasc Res

Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats.

Authors - Xichun Qin
humanWB

Cell Biol Int

Identification and analysis of glycogen synthase kinase 3 beta1 interactome.

Authors - Gao Xuejuan X