验证数据展示
经过测试的应用
Positive WB detected in | CHO cells, HEK-293 cells, mouse liver tissue, rat liver tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MDCK cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15712-1-AP targets GSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, canine, hamster samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat, pig, canine, hamster |
文献引用反应性 | human, mouse, rat, pig, chicken |
免疫原 | GSS fusion protein Ag8338 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glutathione synthetase |
别名 | GSHS, GSH synthetase, GSH S, glutathione synthetase, Glutathione synthase |
计算分子量 | 474 aa, 52 kDa |
观测分子量 | 52 kDa |
GenBank蛋白编号 | BC007927 |
基因名称 | GSS |
Gene ID (NCBI) | 2937 |
RRID | AB_2878171 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P48637 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for GSS antibody 15712-1-AP | Download protocol |
IHC protocol for GSS antibody 15712-1-AP | Download protocol |
IF protocol for GSS antibody 15712-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
ACS Nano Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma | ||
Oxid Med Cell Longev IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer. | ||
Redox Biol γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level. | ||
Pharmacol Res Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming. | ||
FASEB J Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism. | ||