验证数据展示
经过测试的应用
| Positive WB detected in | CHO cells, HEK-293 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Planning an IHC experiment? We recommend our IHCeasy GSS Ready-To-Use IHC Kit. GSS primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15712-1-AP targets GSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, canine, hamster samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat, pig, canine, hamster |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Immunogen | GSS fusion protein Ag8338 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glutathione synthetase |
| Synonyms | GSHS, GSH synthetase, GSH S, glutathione synthetase, Glutathione synthase |
| Calculated Molecular Weight | 474 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC007927 |
| Gene Symbol | GSS |
| Gene ID (NCBI) | 2937 |
| RRID | AB_2878171 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSS antibody 15712-1-AP | Download protocol |
| IHC protocol for GSS antibody 15712-1-AP | Download protocol |
| IF protocol for GSS antibody 15712-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma | ||
Oxid Med Cell Longev IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer. | ||
Redox Biol γ-glutamylcysteine exhibits anti-inflammatory effects by increasing cellular glutathione level. | ||
Pharmacol Res Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming. | ||
FASEB J Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism. | ||