验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse ovary tissue, HeLa cells, mouse kidney tissue, rat liver |
| Positive IHC detected in | mouse liver tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12412-1-AP targets GSTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | GSTM1 fusion protein Ag3113 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glutathione S-transferase mu 1 |
| Synonyms | GST HB subunit 4, GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1 |
| Calculated Molecular Weight | 181 aa, 21 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC024005 |
| Gene Symbol | GSTM1 |
| Gene ID (NCBI) | 2944 |
| RRID | AB_2115925 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09488 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSTM1 antibody 12412-1-AP | Download protocol |
| IHC protocol for GSTM1 antibody 12412-1-AP | Download protocol |
| IF protocol for GSTM1 antibody 12412-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.
| ||
Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation | ||
Acta Neuropathol Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy. | ||
Autophagy ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway. | ||
Virol Sin Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection. |
