验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, mouse ovary tissue, HeLa cells, mouse kidney tissue, rat liver |
Positive IHC detected in | mouse liver tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12412-1-AP targets GSTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | GSTM1 fusion protein Ag3113 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glutathione S-transferase mu 1 |
别名 | GST HB subunit 4, GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1 |
计算分子量 | 181 aa, 21 kDa |
观测分子量 | 27 kDa |
GenBank蛋白编号 | BC024005 |
基因名称 | GSTM1 |
Gene ID (NCBI) | 2944 |
RRID | AB_2115925 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P09488 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for GSTM1 antibody 12412-1-AP | Download protocol |
IHC protocol for GSTM1 antibody 12412-1-AP | Download protocol |
IF protocol for GSTM1 antibody 12412-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Immunity Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.
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Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation | ||
Acta Neuropathol Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy. | ||
Autophagy ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway. | ||
Virol Sin Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection. |