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验证数据展示

  • IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

    IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

    IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66320-1-Ig (same clone as 66320-1-PBS)

    IF Staining of A549 using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • WB analysis using 66320-1-Ig (same clone as 66320-1-PBS)

    WB analysis using 66320-1-Ig (same clone as 66320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

    FC experiment of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (66320-1-Ig, Clone:3F9H8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

Gamma Tubulin Monoclonal antibody, PBS Only

Gamma Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66320-1-PBS

产品说明书

CloneNo. 3F9H8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66320-1-PBS targets Gamma Tubulin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原 Gamma Tubulin fusion protein Ag23913 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin, gamma 1
别名 TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin
计算分子量 51 kDa
观测分子量 48-55 kDa
GenBank蛋白编号BC000619
基因名称 Gamma Tubulin
Gene ID (NCBI) 7283
RRIDAB_2857350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23258
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.