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CoraLite® Plus 555-conjugated Glutamine Synthetase Polyclonal antibody
Glutamine Synthetase Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 555 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-11037 targets Glutamine Synthetase in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Glutamine Synthetase fusion protein Ag1510 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | glutamate-ammonia ligase (glutamine synthetase) |
Synonyms | GLUL, GS, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2 |
Calculated Molecular Weight | 374 aa, 42 kDa |
Observed Molecular Weight | 40-42 kDa |
GenBank Accession Number | BC011700 |
Gene Symbol | Glutamine Synthetase |
Gene ID (NCBI) | 2752 |
Conjugate | CoraLite® Plus 555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 554 nm / 570 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P15104 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLUL(Glutamine synthetase) also named GS and GLNS, belongs to the glutamine synthetase family. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667). Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 555 Glutamine Synthetase antibody CL555-11037 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |