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Glutamine Synthetase Polyclonal antibody

Glutamine Synthetase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat no : 11037-2-AP

Print datasheet

Synonyms

GLUL, GS, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2

验证数据展示

WB analysis using 11037-2-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11037-2-AP
WB result of Glutamine synthetase antibody (11037-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.
IF Staining of mouse brain using 11037-2-AP
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IHC staining of mouse liver using 11037-2-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with GLUL antibody included
IF Staining of mouse liver using 11037-2-AP
Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of Jurkat using 11037-2-AP
1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Glutamine Synthetase Polyclonal antibody (11037-2-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IHC staining of mouse liver using 11037-2-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with GLUL antibody included

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Cat No. 11037-2-AP

Size

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经过测试的应用

Positive WB detected in	Jurkat cells, mouse liver tissue, rat liver tissue
Positive IHC detected in	mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue, mouse brain tissue, human brain tissue
Positive FC (Intra) detected in	Jurkat cells

Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 26 publications below
IHC	See 5 publications below
IF	See 5 publications below
IP	See 1 publications below
ELISA	See 1 publications below

产品信息

11037-2-AP targets Glutamine Synthetase in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited Applications	WB, IHC, IF, IP, ELISA
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	Glutamine Synthetase fusion protein Ag1510 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	glutamate-ammonia ligase (glutamine synthetase)
Synonyms	GLUL, GS, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2
Calculated Molecular Weight	374 aa, 42 kDa
Observed Molecular Weight	40-42 kDa
GenBank Accession Number	BC011700
Gene Symbol	Glutamine Synthetase
Gene ID (NCBI)	2752
RRID	AB_2110650
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15104
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

实验方案

Product Specific Protocols
WB protocol for Glutamine Synthetase antibody 11037-2-AP	Download protocol
IHC protocol for Glutamine Synthetase antibody 11037-2-AP	Download protocol
IF protocol for Glutamine Synthetase antibody 11037-2-AP	Download protocol
FC protocol for Glutamine Synthetase antibody 11037-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IF	Nat Methods Spatially resolved isotope tracing reveals tissue metabolic activity. Authors - Lin Wang View Article
mouse	WB,IHC	Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate Authors - Peng Zhou KD Validated View Article
mouse	WB	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
human,mouse	IF,WB	Protein Cell Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation Authors - Kai-Hui Zhang View Article
human	WB	Sci Transl Med An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs. Authors - Wei-Jian Li View Article
mouse	WB	Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion. Authors - Wei-Feng Cai KD Validated View Article

Glutamine Synthetase Polyclonal antibody

Glutamine Synthetase Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

验证数据展示

WB analysis using 11037-2-AP

WB analysis of Jurkat using 11037-2-AP

IF Staining of mouse brain using 11037-2-AP

IHC staining of mouse liver using 11037-2-AP

IF Staining of mouse liver using 11037-2-AP

FC experiment of Jurkat using 11037-2-AP

IHC staining of mouse liver using 11037-2-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Spatially resolved isotope tracing reveals tissue metabolic activity.

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation

An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

相关产品

Flow Cytometry Perm Buffer (10X)