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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    WB analysis of HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    IP experiment of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    IF Staining of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

HA Tag Monoclonal antibody, PBS Only

HA Tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 66006-2-PBS

产品说明书

CloneNo. 1F5C6

宿主/亚型

Mouse / IgG1

种属反应性

recombinant protein

应用

WB, IF/ICC, IP, ELISA

别名

YPYDVPDYA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66006-2-PBS targets HA Tag in WB, IF/ICC, IP, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 HA Tag
别名 YPYDVPDYA
基因名称 HA Tag
Gene ID (NCBI) 6
RRIDAB_2881490
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.