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HA Tag Monoclonal antibody, PBS Only

HA Tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat no : 66006-2-PBS

Print datasheet

Synonyms

YPYDVPDYA

验证数据展示

  • WB analysis of HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    WB analysis of HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    IP experiment of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    IF Staining of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

产品信息

66006-2-PBS targets HA Tag in WB, IF/ICC, IP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name HA Tag
Synonyms YPYDVPDYA
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.