验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
55224-1-AP targets HCN4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat, hamster |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hyperpolarization activated cyclic nucleotide-gated potassium channel 4 |
| 别名 | HCN4 |
| 计算分子量 | 129 kDa |
| 观测分子量 | 140-160 kDa |
| GenBank蛋白编号 | NM_005477 |
| 基因名称 | HCN4 |
| Gene ID (NCBI) | 10021 |
| RRID | AB_11182714 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3Q4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HCN4 belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. HCN4 may contribute to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP. HCN4 may mediate responses to sour stimuli. (PMID:10228147). Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2). Defects in HCN4 are the cause of Brugada syndrome type 8 (BRS8). This antibody is specific to HCN4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HCN4 antibody 55224-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A A gain-of-function HCN4 mutant in the HCN domain is responsible for inappropriate sinus tachycardia in a Spanish family | ||
iScience Engineering of thick human functional myocardium via static stretching and electrical stimulation. | ||
Bull Exp Biol Med Glucocorticoid Receptor/HCN4 Channels Interaction in Spinal Dorsal Horn Participates in the Regulation of Neuropathic Pain after Peripheral Nerve Injury in Rats | ||
Mol Med Rep G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region | ||
PLoS One DNA methylation cooperates with H3K9me2 at HCN4 promoter to regulate the differentiation of bone marrow mesenchymal stem cells into pacemaker-like cells |