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验证数据展示

  • WB analysis of mouse testis using 16160-1-AP

    WB analysis of mouse testis using 16160-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16160-1-AP

    IF Staining of HepG2 using 16160-1-AP

    Immunofluorescent analysis of HepG2 cells using 16160-1-AP (HDAC1-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IP experiment of mouse testis using 16160-1-AP

    IP experiment of mouse testis using 16160-1-AP

    IP result of anti-HDAC1-specific (IP:16160-1-AP, 4ug; Detection:16160-1-AP 1:500) with mouse testis tissue lysate 4000ug.

HDAC1-specific Polyclonal antibody

HDAC1-specific Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16160-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, mouse brain tissue, mouse thymus tissue
Positive IP detected inmouse testis tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC1 Ready-To-Use IHC Kit. HDAC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16160-1-AP targets HDAC1-specific in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 1
Synonyms GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_004964
Gene Symbol HDAC1
Gene ID (NCBI) 3065
RRIDAB_2118060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13547
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is specific to HDAC1. It does not react with other HDACs.

实验方案

Product Specific Protocols
WB protocol for HDAC1-specific antibody 16160-1-APDownload protocol
IF protocol for HDAC1-specific antibody 16160-1-APDownload protocol
IP protocol for HDAC1-specific antibody 16160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols