验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, mouse brain tissue, mouse thymus tissue |
Positive IP detected in | mouse testis tissue |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16160-1-AP targets HDAC1-specific in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | histone deacetylase 1 |
Synonyms | GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1 |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | NM_004964 |
Gene Symbol | HDAC1 |
Gene ID (NCBI) | 3065 |
RRID | AB_2118060 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13547 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is specific to HDAC1. It does not react with other HDACs.
实验方案
Product Specific Protocols | |
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WB protocol for HDAC1-specific antibody 16160-1-AP | Download protocol |
IF protocol for HDAC1-specific antibody 16160-1-AP | Download protocol |
IP protocol for HDAC1-specific antibody 16160-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |