验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human kidney tissue, MCF-7 cells, rat liver tissue, HeLa cells, HepG2 cells, L02 cells, C6 cells, NIH/3T3 cells, rat kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12922-3-AP targets HDAC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HDAC2 fusion protein Ag3607 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histone deacetylase 2 |
| 别名 | YAF1, RPD3, histone deacetylase 2, HD2, EC:3.5.1.98 |
| 计算分子量 | 458 aa, 52 kDa; 488 aa,55 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC031055 |
| 基因名称 | HDAC2 |
| Gene ID (NCBI) | 3066 |
| RRID | AB_2118516 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92769 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HDAC2 antibody 12922-3-AP | Download protocol |
| IHC protocol for HDAC2 antibody 12922-3-AP | Download protocol |
| IF protocol for HDAC2 antibody 12922-3-AP | Download protocol |
| IP protocol for HDAC2 antibody 12922-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest SAP30 promotes breast tumor progression by bridging the transcriptional corepressor SIN3 complex and MLL1 | ||
Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. | ||
Acta Pharm Sin B PIM1-HDAC2 axis modulates intestinal homeostasis through epigenetic modification | ||
J Clin Invest Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation. | ||
Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression. |
