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验证数据展示

  • WB analysis of K-562 using 12834-1-AP

    WB analysis of K-562 using 12834-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 12834-1-AP

    IP experiment of K-562 using 12834-1-AP

    IP result of anti-HDAC6 (IP:12834-1-AP, 5ug; Detection:12834-1-AP 1:1000) with K-562 cells lysate 11000ug.

  • IHC staining of human testis using 12834-1-AP

    IHC staining of human testis using 12834-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12834-1-AP (HDAC6 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IHC staining of human testis using 12834-1-AP

    IHC staining of human testis using 12834-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12834-1-AP (HDAC6 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HDAC6 antibody included
  • WB analysis using 12834-1-AP

    WB analysis using 12834-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12834-1-AP

    WB analysis of HeLa using 12834-1-AP

    WB result of HDAC6 antibody (12834-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC6 transfected HeLa cells.

  • WB analysis using 12834-1-AP

    WB analysis using 12834-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12834-1-AP (HDAC6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12834-1-AP

    IHC staining of mouse brain using 12834-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12834-1-AP (HDAC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HDAC6 antibody included
  • IF Staining of HepG2 using 12834-1-AP

    IF Staining of HepG2 using 12834-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MG132 treated HepG2 cells using HDAC6 antibody (12834-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 12834-1-AP

    IF Staining of HepG2 using 12834-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12834-1-AP (HDAC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

HDAC6 Polyclonal antibody

HDAC6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12834-1-AP

产品说明书

引用文献(61)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

Protein deacetylase HDAC6, KIAA0901, Histone deacetylase 6, HDAC 6, EC:3.5.1.98

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, human liver tissue, HepG2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue, human lung cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMG132 treated HepG2 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC6 Ready-To-Use IHC Kit. HDAC6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12834-1-AP targets HDAC6 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human
文献引用反应性human, pig, canine
免疫原 HDAC6 fusion protein Ag3572 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone deacetylase 6
别名 Protein deacetylase HDAC6, KIAA0901, Histone deacetylase 6, HDAC 6, EC:3.5.1.98
计算分子量 1063 aa, 114 kDa, 131 kDa
观测分子量 160 kDa
GenBank蛋白编号BC013737
基因名称 HDAC6
Gene ID (NCBI) 10013
RRIDAB_10597094
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBN7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. HDAC6 has some splicing variants such as P114(~130kd), P131(~160kd).This antibody is a rabbit polyclonal antibody raised against residues near the C terminal of human HDAC6. The calculated molecular weight of HDAC6 is 130 kDa, but the modified the HDAC6 is about 150-160 kDa.

实验方案

Product Specific Protocols
WB protocol for HDAC6 antibody 12834-1-APDownload protocol
IHC protocol for HDAC6 antibody 12834-1-APDownload protocol
IF protocol for HDAC6 antibody 12834-1-APDownload protocol
IP protocol for HDAC6 antibody 12834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF,WB

Bioact Mater

Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway

Authors - Tian Qin
View Article
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
View Article
humanWB,IP

Genome Biol

The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots

Authors - Shenli Yuan
View Article
humanWB,IP

Oncogene

HDAC6-G3BP2 promotes lysosomal-TSC2 and suppresses mTORC1 under ETV4 targeting-induced low-lactate stress in non-small cell lung cancer

Authors - Bei Liu
  • KD Validated
View Article
WB

Kidney Int

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Authors - Xiaojie Wang
View Article
humanWB,IHC

Biomed Pharmacother

Degradation of histone deacetylase 6 alleviates ROS-mediated apoptosis in renal ischemia-reperfusion injury

Authors - Kang Xia
View Article