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HDGF Polyclonal antibody

HDGF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11344-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

HMG-1L2, High mobility group protein 1-like 2, Hepatoma-derived growth factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, HEK-293T cells, Hela cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue, human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy HDGF Ready-To-Use IHC Kit. HDGF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11344-1-AP targets HDGF in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human
文献引用反应性human, mouse
免疫原 HDGF fusion protein Ag1893 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hepatoma-derived growth factor (high-mobility group protein 1-like)
别名 HMG-1L2, High mobility group protein 1-like 2, Hepatoma-derived growth factor
计算分子量 26 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC018991
基因名称 HDGF
Gene ID (NCBI) 3068
RRIDAB_2232950
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51858
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDGF, also named as HMG1L2, belongs to the HDGF family. HDGF is a mitogen for many cell types with nuclear localization necessary for its mitogenic activity. HDGF has also been identified as an important prognostic marker in pathologic cell growth, as it is overexpressed in a number of cancers with expression linked to a poor outcome in lung, esophageal, pancreatic and hepatic cancer.HDGF is highly expressed in human NSCLC tissues, predicting worse prognosis in resected NSCLCs. It might be useful molecular biomarker for predicting the prognosis of resected NSCLCs.

实验方案

Product Specific Protocols
WB protocol for HDGF antibody 11344-1-APDownload protocol
IHC protocol for HDGF antibody 11344-1-APDownload protocol
IF protocol for HDGF antibody 11344-1-APDownload protocol
IP protocol for HDGF antibody 11344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Hazard Mater

Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ.

Authors - Ruina Cui
humanIHC,WB

Biomaterials

Gint4.T-siHDGF chimera-capped mesoporous silica nanoparticles encapsulating temozolomide for synergistic glioblastoma therapy

Authors - Huaijun Fei
humanWB,IHC,IF,ChIP

Mol Oncol

Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.

Authors - Xuejie Min
  • KD Validated
humanWB

Front Cell Dev Biol

NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.

Authors - Ye-Wei Zhang
humanWB

Eur J Cancer

Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.

Authors - Li Yan Li YL
humanWB

Hum Cell

LncRNA SNHG3 promotes cell proliferation and invasion through the miR-384/hepatoma-derived growth factor axis in breast cancer.

Authors - Qiuhong Ma
  • KD Validated
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