- Featured Product
- KD/KO Validated
HDGF Polyclonal antibody
HDGF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HEK-293T cells, Hela cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue, human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11344-1-AP targets HDGF in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP, ChIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | HDGF fusion protein Ag1893 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hepatoma-derived growth factor (high-mobility group protein 1-like) |
Synonyms | HMG-1L2, High mobility group protein 1-like 2, Hepatoma-derived growth factor |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | BC018991 |
Gene Symbol | HDGF |
Gene ID (NCBI) | 3068 |
RRID | AB_2232950 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P51858 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HDGF antibody 11344-1-AP | Download protocol |
IHC protocol for HDGF antibody 11344-1-AP | Download protocol |
IF protocol for HDGF antibody 11344-1-AP | Download protocol |
IP protocol for HDGF antibody 11344-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Hazard Mater Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ. | ||
Biomaterials Gint4.T-siHDGF chimera-capped mesoporous silica nanoparticles encapsulating temozolomide for synergistic glioblastoma therapy | ||
Mol Oncol Role of hepatoma-derived growth factor in promoting de novo lipogenesis and tumorigenesis in hepatocellular carcinoma.
| ||
Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma. | ||
Eur J Cancer Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer. | ||
Hum Cell LncRNA SNHG3 promotes cell proliferation and invasion through the miR-384/hepatoma-derived growth factor axis in breast cancer.
|