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HGS Polyclonal antibody
HGS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human liver tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10390-1-AP targets HGS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | HGS fusion protein Ag0589 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hepatocyte growth factor-regulated tyrosine kinase substrate |
Synonyms | ZFYVE8, Protein pp110, Hrs, Hepatocyte growth factor-regulated tyrosine kinase substrate |
Calculated Molecular Weight | 86 kDa |
Observed Molecular Weight | 110 kDa |
GenBank Accession Number | BC003565 |
Gene Symbol | HGS |
Gene ID (NCBI) | 9146 |
RRID | AB_2118914 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14964 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HGS antibody 10390-1-AP | Download protocol |
IHC protocol for HGS antibody 10390-1-AP | Download protocol |
IF protocol for HGS antibody 10390-1-AP | Download protocol |
IP protocol for HGS antibody 10390-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Rep β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development. | ||
Mol Biol Cell The ubiquitin ligase Deltex-3L regulates endosomal sorting of the G protein-coupled receptor CXCR4. | ||
J Cell Sci Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification. | ||
iScience VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion. | ||
iScience Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide. |