验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy HIF1AN Ready-To-Use IHC Kit. HIF1AN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
10646-1-AP targets HIF1AN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | HIF1AN fusion protein Ag0997 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hypoxia inducible factor 1, alpha subunit inhibitor |
| 别名 | Factor inhibiting HIF 1, FIH 1, FIH1, HIF1AN |
| 计算分子量 | 40 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC007719 |
| 基因名称 | HIF1AN |
| Gene ID (NCBI) | 55662 |
| RRID | AB_2232963 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NWT6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1AN (Hypoxia-inducible factor 1-alpha inhibitor) is also named FIH1. It is identified as a molecular oxygen-dependent dioxygenase that blunts the transcriptional activity of HIF and has also been implicated in HIF-dependent and -independent hypoxia responses(PMID:20720525). Structural studies indicate that an FIH-1 homodimer forms in solution and is essential for FIH-1 activity(PMID:16154531).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIF1AN antibody 10646-1-AP | Download protocol |
| IHC protocol for HIF1AN antibody 10646-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Development of a novel RNAi therapy: Engineered miR-31 exosomes promoted the healing of diabetic wounds. | ||
Am J Cancer Res Experimental and clinical evidence suggests that GRPEL2 plays an oncogenic role in HCC development. | ||
Heliyon LncRNA OIP5-AS1 regulates ferroptosis and mitochondrial dysfunction-mediated apoptosis in spinal cord injury by targeting the miR-128-3p/Nrf2 axis |