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HJURP Polyclonal antibody

HJURP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15283-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

FAKTS, FLEG1, hFLEG1, HJURP, Up regulated in lung cancer 9, URLC9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-29 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15283-1-AP targets HJURP in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 HJURP fusion protein Ag7402 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Holliday junction recognition protein
别名 FAKTS, FLEG1, hFLEG1, HJURP, Up regulated in lung cancer 9, URLC9
计算分子量 86 kDa
观测分子量 84 kDa
GenBank蛋白编号BC001940
基因名称 HJURP
Gene ID (NCBI) 55355
RRIDAB_2116879
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NCD3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

实验方案

Product Specific Protocols
WB protocol for HJURP antibody 15283-1-APDownload protocol
IHC protocol for HJURP antibody 15283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

CENP-A Ubiquitylation Is Indispensable to Cell Viability.

Authors - Yohei Niikura
humanIHC

Front Cell Dev Biol

Comprehensive Analysis Uncovers Prognostic and Immunogenic Characteristics of Cellular Senescence for Lung Adenocarcinoma.

Authors - Weihao Lin
humanIHC

Int J Cancer

Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Authors - Fangqiu Fu
humanWB

J Biol Chem

Mitotic regulator Mis18β interacts with and specifies the centromeric assembly of molecular chaperone HJURP.

Authors - Jianyu Wang
  • KD Validated
humanWB

Biochem Biophys Res Commun

Hypomethylation-driven overexpression of HJURP promotes progression of hepatocellular carcinoma and is associated with poor prognosis.

Authors - Ye Li
  • KD Validated

J Vis Exp

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A).

Authors - Yohei Niikura
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