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Hexokinase 2 Polyclonal antibody

Hexokinase 2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22029-1-AP

Print datasheet

Synonyms

Hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase



经过测试的应用

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, Jurkat cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22029-1-AP targets Hexokinase 2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, canine, monkey, chicken, bovine
Immunogen Hexokinase 2 fusion protein Ag16895 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hexokinase 2
Synonyms Hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC021116
Gene Symbol Hexokinase 2
Gene ID (NCBI) 3099
RRIDAB_11182717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52789
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.

实验方案

Product Specific Protocols
WB protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IHC protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IP protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
human,mouseWB

Cell Metab

An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.

Authors - Nunu Huang
mouseWB

Cell Metab

STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth.

Authors - Chunyan Zhang
IP

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
mouseWB

Nat Neurosci

Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.

Authors - Xiaoxiang Dong
human,mouseWB,IHC

Adv Sci (Weinh)

Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis

Authors - Bin Liu