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  • Featured Product
  • KD/KO Validated

HLA class I ABC Monoclonal antibody, PBS Only

HLA class I ABC Monoclonal Antibody for WB, IHC, IF/ICC, FC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

pig, human

Applications

WB, IHC, IF/ICC, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C5B7

Cat no : 66013-1-PBS

Print datasheet

Synonyms

HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A

验证数据展示

  • WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

    WB analysis using 66013-1-Ig (same clone as 66013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-A antibody included
  • IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    IHC staining of human tonsillitis using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HLA-A antibody included
  • FC experiment of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    FC experiment of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed. This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    IF Staining of Raji using 66013-1-Ig (same clone as 66013-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66013-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HLA-A Ready-To-Use IHC Kit. HLA-A primary antibody included.

产品信息

66013-1-PBS targets HLA class I ABC in WB, IHC, IF/ICC, FC, Indirect ELISA applications and shows reactivity with pig, human samples.

Tested Applications WB, IHC, IF/ICC, FC, Indirect ELISA Application Description
Tested Reactivity pig, human
Immunogen HLA class I ABC fusion protein Ag7488 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name major histocompatibility complex, class I, A
Synonyms HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40-44 kDa
GenBank Accession NumberBC003069
Gene Symbol HLA-A
Gene ID (NCBI) 3105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04439
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)