• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

HRC Polyclonal antibody

HRC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18142-1-AP

Print datasheet

Synonyms

Sarcoplasmic reticulum histidine-rich calcium-binding protein

验证数据展示

  • IHC staining of mouse heart using 18142-1-AP

    IHC staining of mouse heart using 18142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse heart using 18142-1-AP

    WB analysis of mouse heart using 18142-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 18142-1-AP

    IHC staining of mouse heart using 18142-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 18142-1-AP

    IF Staining of HeLa using 18142-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, HeLa cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18142-1-AP targets HRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen HRC fusion protein Ag12794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histidine rich calcium binding protein
Synonyms Sarcoplasmic reticulum histidine-rich calcium-binding protein
Calculated Molecular Weight 699 aa, 80 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberBC069795
Gene Symbol HRC
Gene ID (NCBI) 3270
RRIDAB_10598013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HRC antibody 18142-1-APDownload protocol
IHC protocol for HRC antibody 18142-1-APDownload protocol
IF protocol for HRC antibody 18142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Elife

Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy

Authors - Wei Li
View Article
mouseWB

Chem Biol Interact

Vitamin D alleviates liver fibrosis by inhibiting histidine-rich calcium binding protein (HRC).

Authors - Wanyi Lu
View Article
humanWB,IHC

J Adv Res

Inhibition of lung cancer by vitamin D depends on downregulation of histidine-rich calcium-binding protein.

Authors - Ning Liu
  • KO Validated
View Article