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- KD/KO Validated
HRC Polyclonal antibody
HRC Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, HeLa cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18142-1-AP targets HRC in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | HRC fusion protein Ag12794 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | histidine rich calcium binding protein |
| Synonyms | HCP, HRC |
| Calculated Molecular Weight | 699 aa, 80 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC069795 |
| Gene Symbol | HRC |
| Gene ID (NCBI) | 3270 |
| RRID | AB_10598013 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRC antibody 18142-1-AP | Download protocol |
| IHC protocol for HRC antibody 18142-1-AP | Download protocol |
| IF protocol for HRC antibody 18142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy | ||
Chem Biol Interact Vitamin D alleviates liver fibrosis by inhibiting histidine-rich calcium binding protein (HRC). | ||
J Adv Res Inhibition of lung cancer by vitamin D depends on downregulation of histidine-rich calcium-binding protein.
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