• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14700-1-AP

Print datasheet

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

验证数据展示

  • WB analysis using 14700-1-AP

    WB analysis using 14700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14700-1-AP (GRP94 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14700-1-AP

    IF Staining of HepG2 using 14700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRP94 antibody (14700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human breast cancer using 14700-1-AP

    IHC staining of human breast cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of human breast cancer using 14700-1-AP

    IHC staining of human breast cancer using 14700-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14700-1-AP (HSP90B1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IF Staining of A431 using 14700-1-AP

    IF Staining of A431 using 14700-1-AP

    Immunofluorescent analysis of A431 cells, using HSP90B1 antibody 14700-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse liver using 14700-1-AP

    IP experiment of mouse liver using 14700-1-AP

    IP result of anti-GRP94 (IP:14700-1-AP, 4ug; Detection:14700-1-AP 1:2000) with mouse liver tissue lysate 1560 ug.

  • FC experiment of HeLa using 14700-1-AP

    FC experiment of HeLa using 14700-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (14700-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HEK-293 using 14700-1-AP

    WB analysis of HEK-293 using 14700-1-AP

    HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 14700-1-AP (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14700-1-AP

    IF Staining of HeLa using 14700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRP94 antibody (14700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells, A431 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14700-1-AP targets GRP94 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen GRP94 fusion protein Ag6344 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock protein 90kDa beta (Grp94), member 1
Synonyms HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC066656
Gene Symbol GRP94
Gene ID (NCBI) 7184
RRIDAB_2233347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

实验方案

Product Specific Protocols
WB protocol for GRP94 antibody 14700-1-APDownload protocol
IHC protocol for GRP94 antibody 14700-1-APDownload protocol
IF protocol for GRP94 antibody 14700-1-APDownload protocol
IP protocol for GRP94 antibody 14700-1-APDownload protocol
FC protocol for GRP94 antibody 14700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
View Article
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
View Article
humanWB,IF

Theranostics

Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.

Authors - Zhiwei Liao
View Article
ratWB

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang
View Article
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
View Article
mouseWB

Antioxid Redox Signal

PMID: 27565538

Authors - Tianhe Li
View Article