验证数据展示

IHC staining of human breast cancer using 10995-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HSP70 antibody included
WB analysis using 10995-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 10995-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10995-1-AP
IP result of anti-HSP70 (IP:10995-1-AP, 3ug; Detection:10995-1-AP 1:1000) with mouse brain tissue lysate 3600ug.
WB analysis of human urine exosomes using 10995-1-AP
human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 10995-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10995-1-AP (HSP70 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HSP70 antibody included
IHC staining of human colon using 10995-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10995-1-AP (HSP70 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HSP70 antibody included
IF Staining of HeLa using 10995-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP70 antibody (10995-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 10995-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug HSP70 Polyclonal antibody (10995-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO验证

HSP70 Polyclonal antibody

HSP70 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10995-1-AP

产品说明书

引用文献(288)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (288)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, human urine exosomes tissue, A549 cells, HeLa cells, Jurkat cells, mouse brain, HEK-293 cells, mouse kidney, rat kidney
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human normal colon, mouse skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy HSP70 Ready-To-Use IHC Kit. HSP70 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 9 publications below
WB	See 270 publications below
IHC	See 11 publications below
IF	See 23 publications below
IP	See 1 publications below
ELISA	See 1 publications below
FC	See 2 publications below
CoIP	See 5 publications below

产品信息

10995-1-AP targets HSP70 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, ELISA
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig, chicken, zebrafish, sheep, goat, ostrich, oyster
免疫原	HSP70 fusion protein Ag1446 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	heat shock 70kDa protein 1A
别名	HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A
计算分子量	70 kDa
观测分子量	66-70 kDa
GenBank蛋白编号	BC009322
基因名称	HSP70
Gene ID (NCBI)	3303
RRID	AB_2264230
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P0DMV8
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

What is Hsp70/HSP1A?

HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.
FAQs for Hsp70

a. I cannot detect Hsp70 by western blotting

HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

b. What loading control can I use for cellular stress experiments with Hsp70?

Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

实验方案

Product Specific Protocols
WB protocol for HSP70 antibody 10995-1-AP	Download protocol
IHC protocol for HSP70 antibody 10995-1-AP	Download protocol
IF protocol for HSP70 antibody 10995-1-AP	Download protocol
IP protocol for HSP70 antibody 10995-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nature A cytosolic surveillance mechanism activates the mitochondrial UPR Authors - F X Reymond Sutandy View Article
human	WB	Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis Authors - Tian-Yi Jiang View Article
human	WB	Nat Cell Biol Caspase-2 is a condensate-mediated deubiquitinase in protein quality control Authors - Yingwei Ge View Article
human	WB	Nat Cell Biol Heat stress activates YAP/TAZ to induce the heat shock transcriptome. Authors - Min Luo KO Validated View Article
human	WB	Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. Authors - Bing Yao View Article
mouse	WB	Neuron UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model. Authors - Kejing Zhang KD Validated View Article

验证数据展示

IHC staining of human breast cancer using 10995-1-AP

WB analysis using 10995-1-AP

WB analysis using 10995-1-AP

IP experiment of mouse brain using 10995-1-AP

WB analysis of human urine exosomes using 10995-1-AP

IHC staining of human colon using 10995-1-AP

IHC staining of human colon using 10995-1-AP

IF Staining of HeLa using 10995-1-AP

FC experiment of HeLa using 10995-1-AP

HSP70 Polyclonal antibody

HSP70 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A cytosolic surveillance mechanism activates the mitochondrial UPR

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Caspase-2 is a condensate-mediated deubiquitinase in protein quality control

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.