• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

HSP70 Polyclonal antibody

HSP70 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 10995-1-AP

Print datasheet

Synonyms

HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A

验证数据展示

  • IHC staining of human breast cancer using 10995-1-AP

    IHC staining of human breast cancer using 10995-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP70 antibody included
  • WB analysis using 10995-1-AP

    WB analysis using 10995-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10995-1-AP

    WB analysis using 10995-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 10995-1-AP

    IP experiment of mouse brain using 10995-1-AP

    IP result of anti-HSP70 (IP:10995-1-AP, 3ug; Detection:10995-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

  • WB analysis of human urine exosomes using 10995-1-AP

    WB analysis of human urine exosomes using 10995-1-AP

    human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 10995-1-AP

    IHC staining of human colon using 10995-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10995-1-AP (HSP70 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP70 antibody included
  • IHC staining of human colon using 10995-1-AP

    IHC staining of human colon using 10995-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10995-1-AP (HSP70 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP70 antibody included
  • IF Staining of HeLa using 10995-1-AP

    IF Staining of HeLa using 10995-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP70 antibody (10995-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 10995-1-AP

    FC experiment of HeLa using 10995-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HSP70 Polyclonal antibody (10995-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, human urine exosomes tissue, A549 cells, HeLa cells, Jurkat cells, mouse brain, HEK-293 cells, mouse kidney, rat kidney
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human normal colon, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HSP70 Ready-To-Use IHC Kit. HSP70 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10995-1-AP targets HSP70 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ELISA, Cell treatment
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, sheep, goat, ostrich, oyster, c. elegans
Immunogen HSP70 fusion protein Ag1446 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 70kDa protein 1A
Synonyms HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC009322
Gene Symbol HSP70
Gene ID (NCBI) 3303
RRIDAB_2264230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0DMV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

  1. What is Hsp70/HSP1A?

    HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

  2. FAQs for Hsp70

    a. I cannot detect Hsp70 by western blotting

    HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

    b. What loading control can I use for cellular stress experiments with Hsp70?

    Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

实验方案

Product Specific Protocols
WB protocol for HSP70 antibody 10995-1-APDownload protocol
IHC protocol for HSP70 antibody 10995-1-APDownload protocol
IF protocol for HSP70 antibody 10995-1-APDownload protocol
IP protocol for HSP70 antibody 10995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy
View Article
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
View Article
humanWB

Nat Cell Biol

Caspase-2 is a condensate-mediated deubiquitinase in protein quality control

Authors - Yingwei Ge
View Article
humanWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
  • KO Validated
View Article
humanWB

Mol Cancer

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Authors - Bing Yao
View Article
mouseWB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
  • KD Validated
View Article