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  • Featured Product
  • KD/KO Validated

HSP60 Polyclonal antibody

HSP60 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15282-1-AP

Print datasheet

Synonyms

HSPD1, Heat shock protein 60, GROEL, EC:5.6.1.7, CPN60

验证数据展示

  • WB analysis using 15282-1-AP

    WB analysis using 15282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15282-1-AP

    IP experiment of HEK-293 using 15282-1-AP

    IP result of anti-HSP60 (IP:15282-1-AP, 4ug; Detection:15282-1-AP 1:10000) with HEK-293 cells lysate 1800 ug.

  • IF Staining of HeLa using 15282-1-AP

    IF Staining of HeLa using 15282-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (15282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

  • FC experiment of HepG2 using 15282-1-AP

    FC experiment of HepG2 using 15282-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP60 (15282-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • IHC staining of human lung cancer using 15282-1-AP

    IHC staining of human lung cancer using 15282-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPD1 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, NIH/3T3 cells, HeLa, mouse heart, rat heart
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15282-1-AP targets HSP60 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, zebrafish, goat, yeast, fish, duck
Immunogen HSP60 fusion protein Ag7401 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 60kDa protein 1 (chaperonin)
Synonyms HSPD1, Heat shock protein 60, GROEL, EC:5.6.1.7, CPN60
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC003030
Gene Symbol HSP60
Gene ID (NCBI) 3329
RRIDAB_2121440
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

实验方案

Product Specific Protocols
WB protocol for HSP60 antibody 15282-1-APDownload protocol
IHC protocol for HSP60 antibody 15282-1-APDownload protocol
IF protocol for HSP60 antibody 15282-1-APDownload protocol
IP protocol for HSP60 antibody 15282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Authors - Xiaorong Zhang
View Article
mouseWB

Nucleic Acids Res

RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis

Authors - Chao Liu
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mouseWB

J Hazard Mater

Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy

Authors - Jun Wu
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humanWB

Nat Commun

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

Authors - Wenjun Wang
View Article
mouseWB,IF

Nat Commun

Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.

Authors - Gustavo Della-Flora Nunes
View Article
humanWB

Nat Commun

EGFL9 promotes breast cancer metastasis by inducing cMET activation and metabolic reprogramming.

Authors - Fanyan Meng
View Article