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ChromoTek Histone-Label Atto488

Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei. It consists of an anti-histone VHH conjugated to the fluorescent dye ATTO488. For IF, microscopy of histones, chromosomes, and nuclei.

Specificity

Histone H2A-H2B heterodimers

Applications

IF, ICC

Conjugate

ATTO 488

Host

Alpaca

Cat No : tba488

Synonyms

Histone-Label, Histone, Histone H2A, Histone H2B, Histone H2A-H2B

Validation Data Gallery

  • Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

    Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

  • Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

    Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

  • Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

    Hela cells immunostained with Histone-Lable-ATTO488, 1:400. Scale bar, 10 um.

  • ChromoTek Histone-Label is a convenient, ready to use, and high-performing chromatin staining probe with low background levels and that differentiates between euchromatin and heterochromatin. HeLa cells stained in parallel with Histone-Label (1.25 mg/L) and a monoclonal rabbit anti-H2A IgG / anti-rabbit Alexa568 secondary antibody. Histone-Label co-localizes with conventional antibody staining, however Histone-Label better penetrates into the compactly packed nuclei than the anti-H2A IgG and secondary IgGs, which are one order of magnitude larger than the Histone-Label: see more green signal from Histone-Label at center of nuclei and more red signal from anti-H2A IgG on surface/edge of nuclei of merge image on very right.

    ChromoTek Histone-Label is a convenient, ready to use, and high-performing chromatin staining probe with low background levels and that differentiates between euchromatin and heterochromatin. HeLa cells stained in parallel with Histone-Label (1.25 mg/L) and a monoclonal rabbit anti-H2A IgG / anti-rabbit Alexa568 secondary antibody. Histone-Label co-localizes with conventional antibody staining, however Histone-Label better penetrates into the compactly packed nuclei than the anti-H2A IgG and secondary IgGs, which are one order of magnitude larger than the Histone-Label: see more green signal from Histone-Label at center of nuclei and more red signal from anti-H2A IgG on surface/edge of nuclei of merge image on very right.

Product Information

Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei. It consists of an anti-histone VHH conjugated to the fluorescent dye ATTO488. For IF, microscopy of histones, chromosomes, and nuclei.

DescriptionHistone-Label* immunostains chromatin throughout the cell cycle (interphase chromatin, chromatin condensation and mitotic chromosomes) due to specific binding to histone H2A-H2B heterodimers:

• Specific probe for direct immunostaining of histones, chromosomes, and nuclei

• Labels histones throughout the nuclei

• Better penetration into densely packed nuclei

• Higher image resolution

• Monovalent VHHs do not cluster their epitopes

*Commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment
ApplicationsIF, ICC
HostAlpaca
Specificity/TargetHistone-Label immunostains chromatin throughout the cell cycle (interphase chromatin, chromatin condensation and mitotic chromosomes) due to specific binding to histone H2A-H2B heterodimers.
ConjugateATTO 488
Physical StateLiquid
Suggested DilutionIF/ICC: 1:400
Type Nanobody
ClassRecombinant
RRIDAB_2827588
Storage Buffer1x PBS, 0.09% sodium azide.
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for 6 month. Do not freeze. Protect from light.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Commercialized under a license of INRAE, the French National Research Institute for Agriculture, Food and Environment

Publications

ApplicationTitle
IF

Adv Mater

Hydrogel-Induced Cell Membrane Disruptions Enable Direct Cytosolic Delivery of Membrane-Impermeable Cargo.

Authors - Jelter Van Hoeck
View Article
IHC

Nat Biomed Eng

Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies.

Authors - Nobuyuki Tanaka
View Article
IF

Biophys J

DNA length tunes the fluidity of DNA-based condensates.

Authors - Fernando Muzzopappa
View Article
IF

Nano Res

Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation.

Authors - Jing Liu
View Article

JCI Insight

Three-dimensional imaging of upper tract urothelial carcinoma improves diagnostic yield and accuracy

Authors - Keishiro Fukumoto
View Article