Human A1BG ELISA Kit
Cat no : KE00130
Synonyms
Alpha 1B-Glycoprotein, A1B, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein
Validation Data Gallery
Product Information
KE00130 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The A1BG ELISA kit is to be used to detect and quantify protein levels of endogenous A1BG. The assay recognizes human A1BG. An antibody specific for A1BG has been pre-coated onto the microwells. The A1BG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for A1BG is added to detect the captured A1BG protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human A1BG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 21.5 pg/mL |
| Range | 125-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 90%-125% |
| Human plasma | 101% | 77%-122% |
| Cell culture supernatants | 98% | 80%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 257.9 | 14.7 | 5.7 |
| 2 | 20 | 517.3 | 23.3 | 4.5 |
| 3 | 20 | 2,133.3 | 107.2 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 266.1 | 21.2 | 8.0 |
| 2 | 24 | 504.5 | 35.8 | 7.1 |
| 3 | 24 | 2,072.8 | 105.0 | 5.1 |
Background Information
Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. Alpha-1B-glycoprotein contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of alpha-1B-glycoprotein. Alpha-1B-glycoprotein-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3. Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl. Elevated alpha-1B-glycoprotein levels in blood have been associated with several kinds of cancer, including non-small cell lung cancer, endometrial and cervical cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alpha 1B-Glycoprotein, A1B, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancers (Basel) Alpha-1B Glycoprotein Is a Novel Hepatocyte-Derived Host Factor Associated with In Vitro Inhibition of HBV Replication and Hepatocellular Carcinoma Progression. |
