Human APCS ELISA Kit
Cat no : KE00122
Synonyms
SAP, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein, amyloid P component, serum, PTX2
Validation Data Gallery
Product Information
KE00122 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APCS ELISA kit is to be used to detect and quantify protein levels of endogenous APCS. The assay recognizes human APCS. An antibody specific for APCS has been pre-coated onto the microwells. The APCS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for APCS is added to detect the captured APCS protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human APCS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.6 pg/mL |
| Range | 15.6 - 1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 325 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 109% | 90%-121% |
| Human urine | 103% | 75%-119% |
| Cell culture supernatants | 94% | 81%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 24.0 | 2.6 | 10.9 |
| 2 | 20 | 100.4 | 7.1 | 7.1 |
| 3 | 20 | 432.7 | 18.2 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 28.9 | 2.9 | 9.9 |
| 2 | 24 | 106.2 | 10.1 | 9.5 |
| 3 | 24 | 457.7 | 45.0 | 9.8 |
Background Information
Serum amyloid P component (SAP, also known as APCS), belongs to the family of pentraxins, a superfamily of plasma proteins characterized by their pentameric assembly and calcium-dependent ligand binding. SAP is a constitutive serum protein synthesized by hepatocytes. It is structurally related to C-reactive protein, a major acute-phase reactant in humans. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. SAP can also bind to fibrils in all types of amyloid deposits, and contributes to the pathogenesis of amyloidosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SAP, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein, amyloid P component, serum, PTX2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
PLoS One Serum amyloid P component and pro-platelet basic protein in extracellular vesicles or serum are novel markers of liver fibrosis in chronic hepatitis C patients. | ||
Clin Chim Acta Combined analysis of serum SAP and PRSS2 for the differential diagnosis of CD and UC |
