Human APCS ELISA Kit
Cat No : KE00122
Synonyms
9.5S alpha 1 glycoprotein, amyloid P component, serum, APCS, PTX2, SAP, Serum amyloid P component
Validation Data Gallery
Product Information
KE00122 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APCS ELISA kit is to be used to detect and quantify protein levels of endogenous APCS. The assay recognizes human APCS. An antibody specific for APCS has been pre-coated onto the microwells. The APCS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for APCS is added to detect the captured APCS protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human APCS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 2.6 pg/mL |
| Range | 15.6 - 1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 325 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 109% | 90%-121% |
| Human urine | 103% | 75%-119% |
| Cell culture supernatants | 94% | 81%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 24.0 | 2.6 | 10.9 |
| 2 | 20 | 100.4 | 7.1 | 7.1 |
| 3 | 20 | 432.7 | 18.2 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 28.9 | 2.9 | 9.9 |
| 2 | 24 | 106.2 | 10.1 | 9.5 |
| 3 | 24 | 457.7 | 45.0 | 9.8 |
Background Information
Serum amyloid P component (SAP, also known as APCS), belongs to the family of pentraxins, a superfamily of plasma proteins characterized by their pentameric assembly and calcium-dependent ligand binding. SAP is a constitutive serum protein synthesized by hepatocytes. It is structurally related to C-reactive protein, a major acute-phase reactant in humans. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. SAP can also bind to fibrils in all types of amyloid deposits, and contributes to the pathogenesis of amyloidosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 9.5S alpha 1 glycoprotein, amyloid P component, serum, APCS, PTX2, SAP, Serum amyloid P component |
Publications
| Species | Sample Type | Title |
|---|---|---|
PLoS One Serum amyloid P component and pro-platelet basic protein in extracellular vesicles or serum are novel markers of liver fibrosis in chronic hepatitis C patients. | ||
Clin Chim Acta Combined analysis of serum SAP and PRSS2 for the differential diagnosis of CD and UC |