Human Albumin ELISA Kit
Cat No : KE00076
Synonyms
ALB, albumin, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341
Validation Data Gallery
Product Information
KE00076 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Albumin ELISA kit is to be used to detect and quantify protein levels of endogenous Albumin. The assay recognizes human Albumin. An antibody specific for Albumin has been pre-coated onto the microwells. The Albumin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Albumin is added to detect the captured Albumin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Albumin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Saliva, Human milk，Human Cerebrospinal Fluid |
| Assay type | Sandwich |
| Sensitivity | 12.0 ng/mL |
| Range | 50-3200 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 213 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 100% | 93%-109% |
| Saliva | 73% | 62%-80% |
| Human milk | 77% | 62%-86% |
| Human plasma | 85% | 76%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 280.0 | 10.4 | 3.7 |
| 2 | 20 | 1,286.8 | 96.8 | 7.5 |
| 3 | 20 | 2,322.0 | 157.0 | 6.6 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 288.0 | 16.0 | 5.6 |
| 2 | 24 | 1,170.8 | 99.2 | 8.5 |
| 3 | 24 | 2,265.6 | 219.2 | 9.7 |
Background Information
Albumin is the most abundant protein in blood plasma. It is produced by well-differentiated hepatocytes, and plays an important role in maintaining osmotic pressure. The normal range of albumin in plasma is 3.4-5.4 grams per deciliter (g/dL). Decreased levels of serum albumin may be a sign of kidney diseases or liver diseases (like hepatitis and cirrhosis). In additon, glycated serum albumin is also a potential diabetes biomarker. This kit is used to quantify the albumin level.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ALB, albumin, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Biol Sci Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates | ||
Biofabrication Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells. | ||
J Agric Food Chem Optimization of Protein Folding for Improved Secretion of Human Serum Albumin Fusion Proteins in Saccharomyces cerevisiae | ||
Biotechnol Bioeng An integrated biomimetic array chip for establishment of collagen-based 3D primary human hepatocyte model for prediction of clinical drug-induced liver injury. | ||
ACS Appl Bio Mater Development of a 3D Cell Culture System Using Amphiphilic Polydepsipeptides and Its Application to Hepatic Differentiation. | ||
ACS Appl Bio Mater Suspension Culture System for Isolating Cancer Spheroids using Enzymatically Synthesized Cellulose Oligomers |