Human Albumin ELISA Kit
Validation Data Gallery
Product Information
KE00076 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Albumin ELISA kit is to be used to detect and quantify protein levels of endogenous Albumin. The assay recognizes human Albumin. An antibody specific for Albumin has been pre-coated onto the microwells. The Albumin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Albumin is added to detect the captured Albumin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Albumin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Saliva, Human milk，Human Cerebrospinal Fluid 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 12.0 ng/mL |
| Range | 50-3200 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 213 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 100% | 93%-109% |
| Saliva | 73% | 62%-80% |
| Human milk | 77% | 62%-86% |
| Human plasma | 85% | 76%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 280.0 | 10.4 | 3.7 |
| 2 | 20 | 1,286.8 | 96.8 | 7.5 |
| 3 | 20 | 2,322.0 | 157.0 | 6.6 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 288.0 | 16.0 | 5.6 |
| 2 | 24 | 1,170.8 | 99.2 | 8.5 |
| 3 | 24 | 2,265.6 | 219.2 | 9.7 |
Background Information
Albumin is the most abundant protein in blood plasma. It is produced by well-differentiated hepatocytes, and plays an important role in maintaining osmotic pressure. The normal range of albumin in plasma is 3.4-5.4 grams per deciliter (g/dL). Decreased levels of serum albumin may be a sign of kidney diseases or liver diseases (like hepatitis and cirrhosis). In addition, glycated serum albumin is also a potential diabetes biomarker. This kit is used to quantify the albumin level. This kit shows no cross-reactivity with serum of mouse, rat, or bovine origin.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ALB, HSA, Serum albumin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Biol Sci Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates | ||
Biofabrication Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells. | ||
J Agric Food Chem Optimization of Protein Folding for Improved Secretion of Human Serum Albumin Fusion Proteins in Saccharomyces cerevisiae | ||
Commun Biol Development of an AI model for DILI-level prediction using liver organoid brightfield images | ||
Viruses Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection | ||
Biotechnol Bioeng An integrated biomimetic array chip for establishment of collagen-based 3D primary human hepatocyte model for prediction of clinical drug-induced liver injury. |
