Human Angiopoietin 2 ELISA Kit
Validation Data Gallery
Product Information
KE00226 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Angiopoietin 2 ELISA kit is to be used to detect and quantify protein levels of endogenous Angiopoietin 2. The assay recognizes Human Angiopoietin 2. An antibody specific for Angiopoietin 2 has been pre-coated onto the microwells. The Angiopoietin 2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Angiopoietin 2 is added to detect the captured Angiopoietin 2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human Angiopoietin 2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 285 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 100% | 77%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 32.7 | 1.0 | 3.2 |
| 2 | 20 | 122.4 | 4.4 | 3.6 |
| 3 | 20 | 495.2 | 20.4 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 31.6 | 1.3 | 4.1 |
| 2 | 24 | 120.9 | 3.6 | 3.0 |
| 3 | 24 | 512.1 | 23.5 | 4.6 |
Background Information
Angiopoietin 2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin 2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin 2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin 2 is expressed only at sites of vascular remodeling. Angiopoietin 2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGPT2, ANG 2, ANG2, angiopoietin 2, Angiopoietin-2, ANGPT2, PDGFAB |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
