Human Angiopoietin 2 ELISA Kit
Validation Data Gallery
Product Information
KE00226 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Angiopoietin 2 ELISA kit is to be used to detect and quantify protein levels of endogenous Angiopoietin 2. The assay recognizes Human Angiopoietin 2. An antibody specific for Angiopoietin 2 has been pre-coated onto the microwells. The Angiopoietin 2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Angiopoietin 2 is added to detect the captured Angiopoietin 2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human Angiopoietin 2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 4.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 285 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 100% | 77%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 32.7 | 1.0 | 3.2 |
| 2 | 20 | 122.4 | 4.4 | 3.6 |
| 3 | 20 | 495.2 | 20.4 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 31.6 | 1.3 | 4.1 |
| 2 | 24 | 120.9 | 3.6 | 3.0 |
| 3 | 24 | 512.1 | 23.5 | 4.6 |
Background Information
Angiopoietin 2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin 2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin 2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin 2 is expressed only at sites of vascular remodeling. Angiopoietin 2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AGPT2, ANG 2, ANG2, angiopoietin 2, Angiopoietin-2, ANGPT2, PDGFAB |
Publications
| Species | Sample Type | Title |
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