Human CCL4 ELISA Kit
Validation Data Gallery
Product Information
KE00152 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL4 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL4. The assay recognizes human CCL4. An antibody specific for CCL4 has been pre-coated onto the microwells. The CCL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CCL4 is added to detect the captured human CCL4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCL4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 3.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6351 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 75%-121% |
| Cell culture supernatants | 93% | 74%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 436.0 | 38.1 | 8.7 |
| 2 | 20 | 102.2 | 9.8 | 9.6 |
| 3 | 20 | 60.6 | 5.5 | 9.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 417.7 | 28.1 | 6.7 |
| 2 | 24 | 115.5 | 5.7 | 5.0 |
| 3 | 24 | 62.2 | 6.7 | 10.7 |
Background Information
CCL4(C-C chemokine ligand 4), also known as MIP-1β, plays a key role in inflammation and immune regulation. CCL4 is produced by a wide variety of cells including: immune cells (monocytes, B and T cells), fibroblasts, endothelial, and epithelial cells. CCL4 has a broad spectrum of target cells including immature dendritic cells, which express the cognate receptor CCR5. Increased expression of CCL4 was reported in the monocytes and blood of elderly individuals. Serum concentrations of CCL4 have been found to be significantly higher in patients with head and neck squamous cell carcinoma compared with controls.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACT 2, ACT2, CCL4, MIP 1 beta |
Publications
| Species | Sample Type | Title |
|---|---|---|
Adv Sci (Weinh) BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity | ||
Aging (Albany NY) Targeting PRKDC activates the efficacy of antitumor immunity while sensitizing to chemotherapy and targeted therapy in liver hepatocellular carcinoma |