Human CD146/MCAM ELISA Kit
Sensitivity
3.8 ng/mL
Range
62.5-4000 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00065 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCAM ELISA kit is to be used to detect and quantify protein levels of endogenous MCAM. The assay recognizes human MCAM. An antibody specific for MCAM has been pre-coated onto the microwells. The MCAM protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for MCAM is added to detect the captured MCAM protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD146/MCAM ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 3.8 ng/mL |
| Range | 62.5-4000 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4162 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 95% | 80%-125% |
| Human plasma | 92% | 79%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,643.0 | 252.8 | 9.6 |
| 2 | 20 | 487.4 | 40.3 | 8.3 |
| 3 | 20 | 124.3 | 7.3 | 5.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,647.6 | 156.9 | 9.5 |
| 2 | 24 | 344.9 | 27.6 | 8.0 |
| 3 | 24 | 82.4 | 8.1 | 9.8 |
Background Information
MCAM, also known as melanoma cell adhesion molecule (CD146) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. A soluble form of CD146 has also been reported. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD146, MCAM, MUC18 |