Human CD40 ligand/CD154 ELISA Kit
Sensitivity
4.2 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00251
Synonyms
CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP
Validation Data Gallery
Product Information
KE00251 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD40 ligand/CD154 ELISA kit is to be used to detect and quantify protein levels of endogenous CD40 ligand/CD154.The assay recognizes human CD40 ligand/CD154 . An antibody specific for CD40 ligand/CD154 has been pre-coated onto the microwells. The CD40 ligand/CD154 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human CD40 ligand/CD154 is added to detect the captured human CD40 ligand/CD154 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD40 ligand/CD154 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 4.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 959 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 75%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 892.2 | 18.7 | 2.1 |
| 2 | 20 | 211.2 | 6.0 | 2.8 |
| 3 | 20 | 47.0 | 1.7 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 931.2 | 25.5 | 2.7 |
| 2 | 24 | 220.1 | 4.7 | 2.1 |
| 3 | 24 | 51.2 | 3.1 | 6.1 |
Background Information
The CD40 ligand (CD40L), also known as TRAP or CD154, is a member of the TNF superfamily of ligands. CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1. Apart from the 33 kDa transmembrane form, a soluble form of CD40L generated by ectodomain shedding has been reported.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
