Human CD8 Isolation Kit
Cat No : KMS303
CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within immune system. CD8 acts as a coreceptor with T cell receptor on T lymphocyte to recognize antigens displayed by antigen presenting cell in the context MHC II molecules. 10%-30% human lymphocytes are CD8 positive. Human CD8 Isolation Kit is designed to isolate untouched CD8+ T toxicity lymphocytes which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD8+ cells will be retained in the tube by magnetic separator. The untouched CD8+ T toxicity cells are collected in supernatant. Any downstream application can be performed on CD8+ cells after separation, such as Flow Cytometry and in vitro culture.
· MS303A-10: 300 µL Human CD8 Isolation biotin labeled human CD8 selection antibody cocktail (CD4, CD19, CD14, CD56, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)
· MS303B-10: 300µL streptavidin magnetic beads
· MS303A-100: 3mL Human CD8 Isolation biotin labeled human CD8 selection antibody cocktail (CD4, CD19, CD14, CD56, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)
· MS303B-100: 3mL streptavidin magnetic beads
PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1*107 cells.