Human CHI3L1/YKL40 ELISA Kit
Cat No : KE00239
Synonyms
39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40
Validation Data Gallery
Product Information
KE00239 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human CHI3L1/YKL40 ELISA kit is to be used to detect and quantify protein levels of endogenous human CHI3L1/YKL40. The assay recognizes human CHI3L1/YKL40. An antibody specific for human CHI3L1/YKL40 has been pre-coated onto the microwells. The human CHI3L1/YKL40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific forhuman CHI3L1/YKL40 is added to detect the captured human CHI3L1/YKL40 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CHI3L1/YKL40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants , Urine |
| Assay type | Sandwich |
| Sensitivity | 0.39 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1116 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 81%-105% |
| Cell culture supernatants | 87% | 83%-93% |
| Human urine | 93% | 79%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 989.82 | 24.78 | 2.5 |
| 2 | 20 | 239.03 | 8.27 | 3.5 |
| 3 | 20 | 67.01 | 2.07 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,086.40 | 20.08 | 1.9 |
| 2 | 24 | 279.98 | 5.31 | 1.9 |
| 3 | 24 | 71.30 | 3.63 | 5.1 |
Background Information
Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancer Cell PDGFRα+ITGA11+ fibroblasts foster early-stage cancer lymphovascular invasion and lymphatic metastasis via ITGA11-SELE interplay |