Human ECM1 ELISA Kit
Sensitivity
0.1 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00141 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ECM1 ELISA kit is to be used to detect and quantify protein levels of endogenous ECM1. The assay recognizes human ECM1. An antibody specific for ECM1 has been pre-coated onto the microwells. The ECM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for ECM1 is added to detect the captured ECM1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human ECM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1893 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 81%-114% |
| Cell culture supernatants | 85% | 71%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,305.5 | 46.3 | 2.0 |
| 2 | 20 | 615.7 | 24.9 | 4.1 |
| 3 | 20 | 116.9 | 9.4 | 8.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,070.5 | 108.7 | 5.3 |
| 2 | 24 | 572.8 | 29.9 | 5.2 |
| 3 | 24 | 152.8 | 15.9 | 10.4 |
Background Information
Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers. It can be detected in human serum. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ECM1, extracellular matrix protein 1, Secretory component p85 |