Human ENPP-2/Autotaxin ELISA Kit
Cat no : KE00179
Synonyms
ATX, ATX X, AUTOTAXIN, E NPP 2, ENPP2, FLJ26803, LysoPLD, PD IALPHA, PDNP2
Validation Data Gallery
Product Information
KE00179 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ENPP-2/Autotaxin ELISA kit is to be used to detect and quantify protein levels of endogenous ENPP-2/Autotaxin. The assay recognizes human ENPP-2/Autotaxin. An antibody specific for ENPP-2/Autotaxin has been pre-coated onto the microwells. The ENPP-2/Autotaxin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human ENPP-2/Autotaxin is added to detect the captured human ENPP-2/Autotaxin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ENPP-2/Autotaxin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5168 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 93% | 70%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2.53 | 0.09 | 3.5 |
| 2 | 20 | 5.95 | 0.19 | 3.1 |
| 3 | 20 | 19.19 | 0.30 | 1.6 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2.35 | 0.11 | 4.6 |
| 2 | 24 | 5.66 | 0.17 | 3.0 |
| 3 | 24 | 18.53 | 0.48 | 2.6 |
Background Information
ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Biliary atresia patients had greater serum autotaxin and liver stiffness than controls. Serum autotaxin and liver stiffness were markedly elevated in Biliary atresia patients with jaundice compared to those without jaundice. Furthermore, serum autotaxin was correlated with liver stiffness and biochemical parameters in Biliary atresia. This kit is used to quality ENPP2 level in serum.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ATX, ATX X, AUTOTAXIN, E NPP 2, ENPP2, FLJ26803, LysoPLD, PD IALPHA, PDNP2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Pediatr Pulmonol Association between plasma lysophosphatidic acid levels and bronchopulmonary dysplasia in extremely preterm infants: A prospective study | ||
Anal Chim Acta Establishment and clinical application evaluations of a deep mining strategy of plasma proteomics based on nanomaterial protein coronas |
