Human FGF19 ELISA Kit
Validation Data Gallery
Product Information
KE00243 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human FGF19 ELISA kit is to be used to detect and quantify protein levels of endogenous human FGF19. The assay recognizes human FGF19. An antibody specific for human FGF19 has been pre-coated onto the microwells. The human FGF19 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human FGF19 is added to detect the captured human FGF19 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FGF19 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 6.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9965 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 86% | 70%-102% |
| Cell culture supernatants | 100% | 93%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 481.82 | 7.76 | 1.61 |
| 2 | 20 | 116.40 | 1.81 | 1.55 |
| 3 | 20 | 28.77 | 0.98 | 3.42 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 475.73 | 10.87 | 2.29 |
| 2 | 24 | 116.55 | 2.41 | 2.07 |
| 3 | 24 | 27.64 | 1.05 | 3.80 |
Background Information
Fibroblast growth factor 19 (FGF19) is a member of a subfamily of fibroblast growth factors that govern nutrient metabolism. FGF19 is an ileum-derived postprandial enterokine that governs bile acid and nutrient metabolism. Synthesis of FGF19 is up-regulated by bile acids and, conversely, bile acid synthesis is down-regulated by FGF19. FGF19 also controls gallbladder volume. FGF19 binds to a receptor complex composed of the FGF receptor 4 (FGFR4) and a coreceptor called β-Klotho, which are both highly expressed in liver. Binding of FGF19 to the FGFR4-β-Klotho complex results in activation of the small guanosine triphosphatase Ras and extracellular signal-regulated protein kinase (ERK) signaling pathway.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FGF 19, FGF-19, Fibroblast growth factor 19, UNQ334/PRO533 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Prolif Suppression of the gut microbiota-bile acid-FGF19 axis in patients with atrial fibrillation | ||
Inflamm Res Fibroblast growth factor receptor 4 deficiency in macrophages aggravates experimental colitis by promoting M1-polarization | ||
Clin Rheumatol Genetic liability to gut microbiota and inflammatory cytokines in relation to systemic lupus erythematosus risk: a multi-omics study | ||
Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma | ||
Clin Chim Acta Fibroblast growth factor 19 in combination with liver fibrosis markers for non-invasive diagnosis of primary biliary cholangitis-associated cirrhosis. |
