Human FGF2 ELISA Kit
Validation Data Gallery
Product Information
KE00129 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FGF2 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF2. The assay recognizes human FGF2. An antibody specific for FGF2 has been pre-coated onto the microwells. The FGF2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for FGF2 is added to detect the captured FGF2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FGF2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 18.8 pg/mL |
| Range | 31.25-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2247 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 95% | 81%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 507.6 | 25.6 | 5.0 |
| 2 | 20 | 110.7 | 6.3 | 5.7 |
| 3 | 20 | 62.3 | 3.0 | 4.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 500.7 | 21.9 | 4.4 |
| 2 | 24 | 114.3 | 9.8 | 8.6 |
| 3 | 24 | 63.3 | 3.1 | 4.9 |
Background Information
Fibroblast growth factor 2 (FGF2) is one of the most well-studied members of the fibroblast growth factor superfamil. FGF2 can be synthesized and secreted by human adipocytes. FGF2 activates its target receptor tyrosine kinases, the FGFRs, on the cell surface in order to activate numerous downstream pathways, including several mitogen activated protein kinase (MAPK) pathways. FGF2 inhibited TGFβ-mediated fibroblast activation, resulting in more rapidly proliferating, spindle-shaped cells, compared to the more slowly proliferating, flatter TGFβ-treated cells. FGF2 is an important regulator of cell growth and differentiation under physiological and pathological conditions. FGF2 is widely involved in important biological processes such as stem cell proliferation and angiogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Basic fibroblast growth factor, BFGF, FGF2, FGFB, HBGF 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Food Res Int Efficient expression of a cytokine combination in Saccharomyces cerevisiae for cultured meat production | ||
Biotechnol J Evaluation of expression systems for recombinant protein production in chicken egg bioreactors | ||
J Dent Calvaria defect regeneration via human periodontal ligament stem cells and prevascularized scaffolds in athymic rats | ||
ACS Nano Controllably Self-Assembled Antibacterial Nanofibrils Based on Insect Cuticle Protein for Infectious Wound Healing | ||
BMC Cardiovasc Disord Serum fibroblast growth factor-2 levels complement vital biomarkers for diagnosing heart failure | ||
Chin Med Isoforskolin modulates AQP4-SPP1-PIK3C3 related pathway for chronic obstructive pulmonary disease via cAMP signaling |
