Human FLRG ELISA Kit

Sensitivity

4.3 pg/mL

Range

31.25-1000 pg/mL

Reactivity

Human

Cat No : KE00258

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Synonyms

FLRG, Follistatin like protein 3, Follistatin related protein 3, FSRP, FSTL3



Product Information

KE00258 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FLRG ELISA kit is to be used to detect and quantify protein levels of endogenous human FLRG. The assay recognizes human FLRG. An antibody specific for human FLRG has been pre-coated onto the microwells. The human FLRG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human FLRG is added to detect the captured human FLRG protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human FLRG ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine, Human milk
Assay type Sandwich
Sensitivity 4.3 pg/mL
Range 31.25-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 10272

Recovery

Sample TypeAverageRange
Human serum96%80%-106%
Cell culture supernatants93%70%-109%
Urine93%72%-121%
Human milk90%70%-117%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120478.818.03.8
220115.84.13.5
32023.41.98.0

InterAssay

Samplenmean ( pg/mL)SDCV%
124476.712.22.6
224120.62.31.9
32415.81.59.5

Background Information

Follistatin-like 3 (FSTL3), also called follistatin-related protein or follistatin-related gene (FLRG) protein, is a highly conserved monomer secreted glycoprotein located on chromosome 19p13.3. The FSTL3 gene is the target of a novel chromosomal rearrangement discovered by Hayette et al in 1998 in B cells of patients with chronic lymphocytic leukemia. FSTL3 can modulate target gene expression via members of the transforming growth factor β (TGF-β) superfamily. Recent literatures have revealed that FSTL3 regulates multiple biological processes, including cell differentiation, aging, obesity development, arteriosclerosis development, and tumor progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms FLRG, Follistatin like protein 3, Follistatin related protein 3, FSRP, FSTL3