Human/Mouse/Rat GDF-8 ELISA Kit
Validation Data Gallery
Product Information
KE00120 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GDF-8 ELISA kit is to be used to detect and quantify protein levels of endogenous GDF-8. The assay recognizes human,mouse and Rat GDF-8. An antibody specific for GDF-8 has been pre-coated onto the microwells. The GDF-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GDF-8 is added to detect the captured GDF-8 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat GDF-8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 15.6-1000 pg/mL, 31.25-2000 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2660 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 113% | 107%-121% |
| Rat serum | 95% | 75%-117% |
| Cell culture supernatants | 88% | 71%-109% |
| tissue lysates | 93% | 81%-111% |
| Human plasma | 111% | 90%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 52.0 | 1.5 | 2.8 |
| 2 | 20 | 212.4 | 7.2 | 3.4 |
| 3 | 20 | 877.1 | 28.3 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 54.1 | 3.0 | 5.5 |
| 2 | 24 | 217.9 | 6.6 | 3.0 |
| 3 | 24 | 854.3 | 27.4 | 3.2 |
Background Information
Growth Differentiation Factor 8 (GDF-8), also called myostatin, is a member of the transforming growth factor (TGF)-βsuper-family. GDF-8 is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. GDF-8 is also expressed in the human reproductive system, such as in granulosa cells, follicular fluid and trophoblasts. In addition, GDF-8 has also functions in heart and adipose tissue, and is related to interstitial fibrosis in the heart, human cancer cachexia, and activation of inflammatory cytokines and insulin resistance.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GDF8, GDF8/Myostatin, MSTN, myostatin, GDF 8 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Br J Pharmacol A new therapeutic effect of fenofibrate in Duchenne muscular dystrophy: The promotion of myostatin degradation. | ||
Nutrition Viral eradication by direct-acting antivirals does not decrease the serum myostatin level in patients infected with hepatitis C virus. | ||
Integr Med Res Chinese herbal medicine (Bu-Shen-Tian-Jing Formula) for outcomes of IVF in Chinese patients with polycystic ovary syndrome: a retrospective cohort study. | ||
J Cachexia Sarcopenia Muscle Fibro-Adipogenic Progenitors Regulate Orofacial Neuromuscular Junction Regeneration via Myostatin. |
